Phospho-CHEK2 (Thr68) Polyclonal Antibody (E-AB-20843)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human uterus Verified Samples in IF: Rat lung |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68 |
| Synonyms | CDS 1, CHEK 2, CHK2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), Cds1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, Chek2, Chk 2, HuCds 1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Ser, Serine/threonine protein kinase Chk2, hCds1 |
| Swissprot | |
| Calculated MW | 61 kDa |
| Observed MW |
61 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Nucleus, Isoform 10 is present throughout the cell and Nucleus>PML body, Nucleus>nucleoplasm, Recruited into PML bodies together with TP53. |
| Tissue Specificity | High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. |
Other Clones
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Other Formats
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Unconjugated
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