Phosphatidylcholine (PC) Colorimetric Assay Kit (E-BC-K796-M)
For research use only.
| Detection Principle |
Phosphatidylcholine (PC) is an important component of eukaryotic cell membranes and a major source of second messenger diacylglycerol, phosphatidic acid, lysophosphatidic acid, and arachidonic acid, which can be further metabolized into other signaling molecules. It is widely found in a variety of foods, especially egg yolks, soy, fish, meat, and some seeds, and it can also be consumed through dietary supplements. PC provides structural stability and fluidity of cell membranes. In the liver, PC is involved in lipid synthesis and transport, helping to maintain lipid balance. As an important component of bile, PC promotes the digestion and absorption of lipid. PC supplements are used to intervene and treat liver diseases such as fatty liver disease and cirrhosis, and are used by athletes to improve physical fitness and endurance. Therefore, the detection of PC has important application value in medical diagnosis, disease management and health evaluation. The detection principle of this kit: Enzyme catalyze the PC and chromogenic agent to produce, with maximum absorption at 550 nm, and the content of PC in the sample can be calculated by measuring the OD value at 550 nm. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-560 nm, optimum wavelength: 550 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.003 mmol/L |
| Detection Range | 0.003-0.500 mmol/L |
| Precision | inter-assay CV: 2.7-9.9% | intra-assay CV: 2.0-2.1% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| Mouse serum | 1-3 |
| Mouse plasma | 1-3 |
| Rat serum | 1-3 |
| Rat plasma | 1-3 |
| Human serum | 1-3 |
| 10% Rose petal tissue homogenate | 1 |
| 10% Corn tissue homogenate | 1 |
| 10% Ligustrum leaf tissue homogenate | 1 |
| 10% Garlic tissue homogenate | 1 |
| 1×10^6 293T cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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