Phenylalanine Ammonia Lyase (PAL) Activity Assay Kit (E-BC-K522-S)
For research use only.
| Detection Principle | Phenylalanine ammonia lyase (PAL) can catalyze L-phenylalanine to produce trans-cinnamic acid and ammonia, and trans-cinnamic acid has the maximum absorption peak at 290 nm. PAL activity can be calculated by measuring the increase of OD value at 290 nm. |
| Synonyms | PAL |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (290 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.78 U/g tissue |
| Detection Range | 0.78-156 U/g tissue |
| Precision | inter-assay CV: 4.6% | intra-assay CV: 3.1% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Epipremnum aureum tissue homogenate | 1 |
| 10% Carrot tissue homogenate | 1 |
| 10% Green pepper tissue homogenate | 1 |
| 10% Corn grain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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