PGFRA Polyclonal Antibody (AN006950L)
For research use only.
| Verified Samples | Verified Samples in WB: SH-SY5Y, C6, NIH/3T3 |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human PGFRA protein expressed by E.coli |
| Abbre | PGFRA |
| Synonyms | GAS, PDGFRA, CD140A, PDGFR-2, PDGFR2, GAS9, RHEPDGFRA, PDGF-R&, alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD_antigen: CD140a, CD140 antigen-like family member A, CD140a antigen, MGC74795, PDGF alpha chain, PDGFR 2, PDGFR alpha, PDGFRA/BCR fusion, PDGFR-alpha, PDGF-R-alpha, PGFRA, Platelet derived growth factor receptor, Platelet derived growth factor receptor 2, Platelet derived growth factor receptor alpha, Platelet derived growth factor receptor alpha polypeptide, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, platelet-derived growth factor receptor A, Platelet-derived growth factor receptor alpha, platelet-derived growth factor receptor α, Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein |
| Swissprot | |
| Calculated MW | 123 kDa |
| Observed MW |
150 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Cell projection, Golgi apparatus |
| Tissue Specificity | Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cardiovascular, Signal Transduction, Cancer |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. |
Other Clones
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Other Formats
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Unconjugated
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