Peroxidase (POD) Activity Assay Kit (Plant samples) (E-BC-K227-M)
For research use only.
| Detection Principle | The peroxidase can catalyze the decomposition of H2O2 and produce water and oxygen. And oxygen oxidized pyrogallic acid to form yellow product. The activity of peroxidase can be calculated by measuring the absorbance at 420 nm. |
| Synonyms | POD |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (410-430 nm, optimum wavelength: 420 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 U/mL |
| Detection Range | 0.01-100 U/mL |
| Precision | inter-assay CV: 0.05 | intra-assay CV: 3.2% |
| Sample Volume | 40 μL(tissue homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Green pepper tissue homogenate | 1 |
| 10% Chive leaf tissue homogenate | 1 |
| 10% Photinia leaf tissue homogenate | 1 |
| 10% Epipremnum aureum tissue homogenate | 1 |
| 10% Mushrooms tissue homogenate | 1 |
| 10% White radish tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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