Pepsin Activity Assay Kit (E-BC-K844-M)

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	96T	$ 320.00

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Detection Principle	Pepsin can catalyze substrate hydrolysis, and the hydrolysate reacts with the chromogenic agent under alkaline conditions to form a blue substance. The blue substance has a maximum absorption peak at 660 nm, and the color depth is proportional to the activity of pepsin. The enzyme activity can be calculated by OD value.
Sample Type	Gastric,Intestinal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (650-670 nm, optimum wavelength: 660 nm)
Research Area	Amino Acids And Proteins
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.63 U/mL
Detection Range	0.63-94.98 U/mL
Precision	inter-assay CV: 4.8-5.6% \| intra-assay CV: 1.3-2.2%
Sample Volume	80 μL(tissue homogenate)

Cat.No.	Product Name	Sizes
E-BC-K843-M	Trypsin Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
20% Rat gastric tissue homogenate	1
20% Mouse gastric tissue homogenate	1
20% Rat intestinal tissue homogenate	1
20% Mouse intestinal tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.