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100μL $ 320.00
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For research use only.

Verified Samples Verified Samples in WB: A431
Dilution WB 1:500-1:1000,  
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB
Clonality Polyclonal
Immunogen A synthetic peptide corresponding to the center region of the human PAK1
Abbre PAK1
Synonyms PAK,  PAK1,  PAKalpha,  ADRB2,  Alpha PAK,  Alpha-PAK,  MGC130000,  MGC130001,  p21 activated kinase 1,  p21 protein (Cdc42/Rac) activated kinase 1,  p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related),  p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog,  p21-activated kinase 1,  p65 PAK,  p65-PAK,  P68-PAK,  PAK alpha,  PAK-1,  Paka,  Protein kinase MUK2,  Rac/p21-activated kinase,  Serine/threonine-protein kinase PAK 1,  STE20 homolog yeast,  yeast)
Swissprot
Calculated MW 61 kDa
Observed MW 61 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1 mg/mL
Buffer 0.2 μm filtered solution in PBS
Purification Method Protein A & Antigen Affinity
Research Areas Cell Biology,  Signal Transduction,  Cancer,  Microbiology,  Neuroscience
Conjugation Unconjugated
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Shipping Ice bag
background This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc39 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Other Clones

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Unconjugated

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