Oxalate (Oxalic Acid) Colorimetric Assay Kit (Extraction method) (E-BC-K1106-M)
For research use only.
| Detection Principle |
Oxalic, also known as ethanedioic acid, is a simple organic acid in plants and plays important physiological roles. Oxalic participates in the regulation of Ca2+, pH and osmotic pressure within cells, as well as the defense responses of plant pathogens and the alleviation of aluminum toxicity. Although Oxalic has important physiological functions, excessive intake of Oxalic can also cause many adverse effects on the human body. Oxalic affects the absorption of mineral elements such as calcium and iron by the human body, increases the risk of developing kidney stones, inflammation, hyperoxaluria, uremia and other diseases, and in severe cases, it can even cause acute Oxalic poisoning. Moreover, oxalic is not easily oxidized and decomposed in the human body, and its metabolites can disrupt the acid-base balance in the body. The detection principle of this kit: Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. Hydrogen peroxide reacts with chromogenic substances under the action of enzymes to form colored products. There is a maximum absorption peak at 550 nm, and the color depth is proportional to the content of oxalic. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (545-555 nm, optimum wavelength: 550 nm) |
| Research Area | Liver And Renal Function , Plant Physiology Series |
| Other Reagents Required | Absolute ethanol |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.006 mmol/L |
| Detection Range | 0.006-1 mmol/L |
| Precision | inter-assay CV: 4.5-7.2% | intra-assay CV: 2.1-3.2% |
| Sample Volume | 500 μL |
| Assay Time | 2 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Spinach tissue homogenate | 1 |
| 10% Pumpkin tissue homogenate | 1 |
| Human urine | 1 |
| Rat urine | 1 |
| Human serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® 430
Elab Fluor® 488
Elab Fluor® 568
Elab Fluor® 594
Elab Fluor® 647
Elab Fluor® 680
Elab Fluor® 700
Elab Fluor® 750
Elab Fluor® Red 780
Elab Fluor® Violet 450
Elab Fluor®488
Elab Fluor®594
Elab Fluor®647
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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