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Oxalate (Oxalic Acid) Colorimetric Assay Kit (E-BC-K892-M)

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96T $ 455.00
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For research use only.

Detection Principle Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. Under the action of POD, hydrogen peroxide reacts with chromogenic substances to produce colored products. There is a specific absorption peak at 550 nm, and the color depth is proportional to the content of oxalate.
Sample Type Serum,Plasma,Plant tissue
Detection Method Colorimetric method
Detection Instrument Micropipettor, Microplate reader (545-555 nm, optimum wavelength: 550 nm)
Research Area Liver And Renal Function ,  Plant Physiology Series
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.02 mmol/L
Detection Range 0.02-1 mmol/L
Precision inter-assay CV: 4.7% | intra-assay CV: 3.0%
Sample Volume 20 μL
Assay Time 35 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human urine 1-2
Human plasma 1
10% Epipremnum aureum tissue homogenate 2-3
Rats plasma 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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