For research use only.
| Verified Samples | Verified Samples in WB: 293T, K562 Verified Samples in IHC: Human liver cancer |
| Dilution | WB 1:500-1:2000, IHC 1:25-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human NUDT19 |
| Abbre | NUDT19 |
| Synonyms | mitochondrial, NUD19, NUDT19, Nucleoside diphosphate-linked moiety X motif 19, Nudix motif 19, RP2, mitochondrial, nucleoside diphosphate-linked moiety X motif 19, nudix (nucleoside diphosphate linked moiety X)-type motif 19 |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW | Refer to figures The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Peroxisome. Mitochondrion. |
| Concentration | 0.9 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Metabolism, Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | NUDT19 (nudix (nucleoside diphosphate linked moiety X)-type motif 19), also known as RP2, is a 375 amino acid protein and coenzyme A diphosphatase that assists in the hydrolysis of CoA esters. A member of the nudix hydrolase family, NUDT19 contains one nudix hydrolase domain and localizes to both mitochondria and peroxisome. NUDT19 utilizes magnesium and/or manganese as cofactors and is encoded by a gene that maps to human chromosome 19q13.11. Chromosome 19 consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. |
Other Clones
1 Results
Other Formats
1 Results
Unconjugated
