NRAS/N-Ras Monoclonal Antibody (AN200076P)
For research use only.
| Verified Samples | Verified Samples in WB: A431, Mouse brain |
| Dilution | WB 1:500-1:2000, |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human NRAS / N-Ras Protein |
| Abbre | NRAS |
| Synonyms | ALPS, HRAS, NRAS, ALPS4, CMNS, N-ras, NCMS, NRAS1, NS6, GTPase NRas, GTPase, HRAS1, RASN, Transforming protein N-Ras, AV095280, N ras, N ras protein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHUMP00000013879, OTTMUSP00000023521, Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog |
| Swissprot | |
| Calculated MW | 21 kDa |
| Observed MW |
21 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Golgi apparatus membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Tags & Cell Markers |
| Clone No. | 5B12 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011] |
Other Clones
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Unconjugated
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