Neutrophil Elastase (NE) Activity Colorimetric Assay Kit (E-BC-K899-M)
For research use only.
| Detection Principle |
Neutrophil Elastase (NE) is one of the main serine proteases secreted by neutrophils. It plays an important role in the physiological and pathological processes of the body, on the one hand NE participate in the formation of the body's defense system and assist in the elimination of pathogenic microorganisms. On the other hand, the excessive activation of NE, in the case of imbalance with endogenous inhibitors, is directly involved in tissue damage, and is closely related to the occurrence of a variety of inflammation-related diseases, such as atherosclerosis, chronic obstructive pulmonary disease, pulmonary cystic fibrosis and other diseases, which can be used as a prevention and treatment target for these diseases. The detection principle of this kit is as follows: NE hydrolyzates the substrate to generate chromogenicity substances, which have maximum absorption at the wavelength of 380 nm, and NE activity in the sample is calculated by measuring the OD value at 380 nm and the standard curve. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (370-390 nm, optimum wavelength: 380 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.54 U/L |
| Detection Range | 0.54-28.57 U/L |
| Precision | inter-assay CV: 6.3-7.5% | intra-assay CV: 4.1-5.0% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse Pancreas tissue homogenate | 5-15 |
| 10% Mouse Small intestine tissue homogenate | 1 |
| 10% Mouse Stomach tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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