Neuraminidase (NA) Activity Fluorometric Assay Kit (E-BC-F083)
For research use only.
| Detection Principle | Neuraminidase (NA), also known as sialase, is a family of glycolipid, oligosaccharide and glycoprotein terminal sialic acid residues that can be hydrolyzed, distributed in viruses, bacteria, fungi and fungi. In vertebrate cells. The detection principle of the test box is to calculate the neuraminidase activity of the sample by detecting the production rate of fluorescent substances per unit time. |
| Sample Type | Animal tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=330 nm/450 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Isopropyl alcohol (AR) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.92 U/L |
| Detection Range | 1.92-7.97 U/L |
| Precision | inter-assay CV: 5.0-10.0% | intra-assay CV: 2.2-5.0% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1-2 |
| 10% Mouse heart tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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