NDUFB10 Polyclonal Antibody (E-AB-32179)
For research use only.
| Verified Samples |
Verified Samples in WB: COLO205 |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-p |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from the Internal region of human NDUFB10 |
| Abbre | NDUFB10 |
| Synonyms | 0610011B04Rik, 22kDa, CI PDSW, CI-PDSW, Complex I PDSW, Complex I PDSW subunit, Complex I-PDSW, NA, NADH dehydrogenase (ubiquinone) 1 beta subcomplex 10 22kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NADH ubiquinone oxidoreductase PDSW subunit |
| Swissprot | |
| Calculated MW | 21 kDa |
| Observed MW |
24 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion inner membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 is an enzyme that in humans is encoded by theNDUFB10 gene.NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 10 is an accessory subunit of the NADH dehydrogenase (ubiquinone) complex, located in the mitochondrial inner membrane.It is also known as Complex I and is the largest of the five complexes of the electron transport chain. |
Other Clones
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Unconjugated
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