NANOS1 Polyclonal Antibody (E-AB-19439)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse muscle |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human NANOS1 |
| Abbre | NANOS1 |
| Synonyms | EC Rep1a, EC_Rep1a, NANO1, NANOS 1, NANOS1, NOS-1, Nanos homolog 1, Nanos homolog 1 (Drosophila), Nos 1, Nos1, OTTHUMP00000046853, Rep 1a, Rep1a |
| Swissprot | |
| Calculated MW | 30 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm>perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Epigenetics and Nuclear Signaling, Developmental Biology, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | NANOS1 (Nanos C2HC-Type Zinc Finger 1) is a Protein Coding gene. Diseases associated with NANOS1 include Spermatogenic Failure 12. GO annotations related to this gene include RNA binding and translation repressor activity. An important paralog of this gene is NANOS3.This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. |
Other Clones
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Unconjugated
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