NADPH Oxidase (NAO) Activity Colorimetric Assay Kit (E-BC-K815-M)
For research use only.
| Detection Principle |
NADPH Oxidase (NAO) is a membrane protein mainly found in mammalian neutrophils, plant cells and filamentous fungal cells, and was initially discovered in phagocytic cell membranes. NAO can oxidize NADPH to NAD+ while generating superoxide anions, and it is one of the main sources of reactive oxygen species (ROS) in the body. NAO is involved in many important physiological processes, including host defense, post-translational modification of proteins, cell signal transduction, gene expression regulation and cell differentiation. The detection principle of this kit is as follows: NAO catalyzes the substrate to produce product, which react with chromogenic agent to form chromogenic substance. It has the maximum absorption at 450 nm. The enzyme activity of NAO is calculated by measuring the OD value at 450 nm. |
| Sample Type | Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Ferroptosis , Cuproptosis , Disulfidptosis , Metabolic Diseases , Oxidative Stress |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.27 U/L |
| Detection Range | 0.27-43.43 U/L |
| Precision | inter-assay CV: 5.0-9.5% | intra-assay CV: 2.7-4.1% |
| Sample Volume | 40 μL(tissue/cell homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 2-10 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Potato tissue homogenate | 1 |
| 10% Corn tissue homogenate | 1 |
| 10% Garlic tissue homogenate | 1 |
| 10% Broccoli tissue homogenate | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 RAW 264.7 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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