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NADPH oxidase 4 (NOX4) Polyclonal Antibody (E-AB-93356)

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AllSizePriceQty
200μL $ 530.00
120μL $ 320.00
60μL $ 200.00
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For research use only.

Verified Samples Verified Samples in WB: various cell lines, various cell lines
Verified Samples in IHC: Rat lung, Rat kidney
Dilution WB 1:500-1:2000,  IHC 1:50-1:100
Isotype IgG
Host Rabbit
Reactivity Mouse,  Rat
Applications WB,  IHC
ClonalityPolyclonal
ImmunogenRecombinant fusion protein of human NADPH oxidase 4
AbbreNADPH oxidase 4 (NOX4)
SynonymsKOX,  KOX-1,  NOX4,  RENOX
Swissprot
Calculated MW6 kDa/25-31 kDa/58-66 kDa
Observed MW 67 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular LocalizationCell junction, Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein, Nucleus, focal adhesion, nucleolus.
Concentration1 mg/mL
BufferPhosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification MethodAffinity purification
Research AreasCancer,  Cell Biology,  Cardiovascular,  Metabolism,  Signal Transduction
ConjugationUnconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
ShippingThe product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
backgroundThis gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
Other Clones

1 Results

    Other Formats

    1 Results

    Unconjugated

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