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NA/NE(Noradrenaline/Norepinephrine) ELISA Kit (E-EL-0047)

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AllSizePriceQty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only. Order now, ship in 3 days

Product Summary
Sensitivity0.19 ng/mL
Detection Range0.31-20 ng/mL
Sample Volume50 μL
Total Assay Time2 h 30 min
ReacitivityUniversal
SpecificityThis kit recognizes Universal NA/NE in samples.No significant cross-reactivity or interference between Universal NA/NE and analogues was observed
Recovery80%-120%
Sample TypeSerum, plasma and other biological fluids
Detection MethodColorimetric method, ELISA, Competitive
Assay TypeCompetitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage2-8℃
Expiration Date12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal NA/NE. During the reaction, Universal NA/NE in the sample or standard competes with a fixed amount of Universal NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal NA/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal NA/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Norepinephrine (INN name: Norepinephrine, also known as Noradrenaline, abbreviated NE or NA), also known as norepinephrine, is a substance formed after the removal of N-methyl group of adrenaline, and also belongs to catecholamines in chemical structure. It is both a neurotransmitter, mainly synthesized and secreted by sympathetic postganglionic neurons and adrenergic nerve endings in the brain, which is the main transmitter released by the latter, and a hormone, synthesized and secreted by the adrenal medulla, but in smaller amounts.
Research Area Neuroscience, Cardiovascular
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      1 Results

        • Q1:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?

          Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.

        • Q2:Which variant of the spike protein does this kit detect? Do you have a kit specific to detect the spike protein for the omicron variant?

          This kit is designed for the original strain of the new crown virus, and the omicron variant has not been verified. However, we have verified 26 recombinant variants of the SARS-CoV-2 spike protein through the kit. For more information, customers can refer to the kit instructions (https://file.elabscience.com/Manual/covid_19/E-EL-E605 .pdf).

        • Q3:What is the range of enzyme activity of your IL-2 freeze-dried powder

          Currently our freeze-dried powder is a concentration unit with no information on the activity unit for the time being.

        • Q4:What is the principle of adding stop solution to stop color reaction in ELISA experiment?

          On the one hand, the activity of HRP enzyme is destroyed and the catalytic function of HRP enzyme is lost. On the other hand, the final color changes due to a change in pH.

        • Q5:The absorbance of the cell supernatant is less than that of the sample diluent alone, so how to concentrate the sample?

          The amount of medium can be reduced for subsequent drug administration and modeling.

        • Q6:My sample volume is small. Can I reduce some reagents proportionally?

          No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.

        • Q7:May I ask which type of plate to choose when ELISA testing?

          According to the different bottom, it is divided into flat bottom, U-shaped bottom, V-shaped bottom and so on. The index of refraction of the flat bottom is low, which is suitable for detection in the enzyme reader. According to the color can be divided into transparent, black, white. Transparent is commonly used for the most general enzyme-linked immunoassay.

        • Q8:Is the TGF-β1 ELISA Kit detecting the active form of TGF-β1 or the precursor?

          The active TGF-β dimer was detected

        • Q9:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?

          Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.

        • Q10:I got nothing on the IL-18 standard

          The standard product is placed in the reagent bottle and then freeze-dried. You can first centrifuge the reagent bottle with 10000×g for 1min, and then directly observe the bottom or side wall of the reagent bottle.