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For research use only.
| Detection Principle | β-N-Acetylglucosaminidase (NAG) catalyzed the substrate to produce p-nitrophenol, which has an absorption wavelength at 400 nm. Therefore, the activity of NAG can be calculated by measuring the change of absorbance value at 400 nm. |
| Synonyms | NAG |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (390-415 nm, optimum wavelength: 400 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.76 U/L |
| Detection Range | 0.76-49.51 U/L |
| Precision | inter-assay CV: 4.6-6.8% | intra-assay CV: 3.2-5.0% |
| Sample Volume | 40 μL |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 3-5 |
| 10% Mouse kidney tissue homogenate | 3-5 |
| 10% Mouse heart tissue homogenate | 3-5 |
| 10% Mouse lung tissue homogenate | 3-5 |
| Rat plasma | 1 |
| Human serum | 1 |
| Bovine serum | 2-3 |
| 6 × 10^6 CHO cells | 1 |
| 0.6 × 10^6 293T/17 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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