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Myeloperoxidase (MPO) Activity Assay Kit (E-BC-K074-S)

All Size Price Qty
100Assays $ 180.00
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For research use only.

Detection Principle Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.
Synonyms MPO
Sample Type Serum,Plasma,Milk,Animal tissue,Cell
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (460 nm)
Research Area Oxidative Stress
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 16.95 U/L
Detection Range 16.95-3349 U/L
Precision inter-assay CV: 9.8% | intra-assay CV: 4.5%
Sample Volume 450-900 μL
Assay Time 1 h 30 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Human plasma 1
Rat serum 1
5% Mouse brain tissue homogenization 1
5% Mouse heart tissue homogenization 1
Human milk 1
Human serum 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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