For research use only. Order now, ship in 3 days
| Product Introduction | Mycoplasma is the smallest and simplest prokaryote found so far, with a diameter of 0.1-0.3 μm. It can easily pass through the filter membrane and enter the cell culture system. It has various forms such as sphere, rod, filamentous and branching. Usually attached to the surface of the cell membrane, there is no cell wall, so ordinary antibiotics have no effect on it. Mycoplasma contamination is widespread and often underestimated in cell cultures. Mycoplasma contamination can cause many problems, such as changes in cell growth rate, cell morphology, chromosome aberrations, cell membrane antigenicity changes, cell metabolism changes, reduced survival rate after cell resuscitation, and so on, which ultimately leads to inaccurate or incorrect experimental results. Therefore, it is of great significance to clean up mycoplasma contamination in cell culture. |
| Concentration | 1× |
| Format | Liquid |
| Mycoplasmal Detection | Negative |
| pH | 7.2-7.5 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Endotoxin Level | ＜3 |
| Storage | This product can be stored at 2-8℃ for 6 months with shading light. |
| Shipping | Ice bag |
| Expiration Date | 6 months |
| Matters Need Attention | 1. This product is for research use only. 2. This product is sterilized by 0.1 μm filtration. 3. It is necessary to pay attention to the aseptic operation and avoid the contamination. |
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Q1:Will improper storage of my culture reagents affect the growth rate of my culture?
Yes. Store animal sera at -5 to -20℃. Store media at 2 to 8℃; use within recommended shelf life period. Store complete media (supplemented) at 2 to 8℃, and for complete medium the recommended shelf life is 3 to 6 months. Additionally, minimize exposure of sera and media to light.
Q2:Where can I find the osmolality for Medium?
The osmolality is listed in the COA for the particular lot number of the medium.
Q3:My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Q4:I see a precipitate in my medium, and a change in the pH. What should I do?
This is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
Q5:How long can I keep my media after supplementing with serum?
Generally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
Q6:How light sensitive is media? Should I also be protecting it from LED light?
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
Q7:Do you provide customized culture media services?
Yes. We can customize culture media with reduced components or changes in the concentration of existing components.
