Mouse sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit (E-EL-M1129)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sC5b-9. You can calculate the concentration of Mouse sC5b-9 in the samples by comparing the OD of the samples to the standard curve.

Soluble Terminal Complement Complex C5b-9 is an important component of the complement system, which plays a crucial role in the immune system's defense mechanism. The complement system is a group of proteins that work together to act on the outer membranes of bacteria and other microorganisms, causing their destruction and death. The C5b-9 complex is the end product of complement activation and is capable of forming pores that destroy the integrity of cells and cause fatal damage to pathogens.