Mouse IL-17RA (Interleukin-17 receptor A) ELISA Kit

Size	Price	Qty
96T	$ 495.00
48T	$ 396.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Sensitivity	9.83 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes IL-17RA in samples.No significant cross-reactivity or interference between IL-17RA and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-17RA . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-17RA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-17RA , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IL-17RA . You can calculate the concentration of IL-17RA in the samples by comparing the OD of the samples to the standard curve.

Background

Six interleukin-17 (IL-17)-family (IL-17A-IL-17F) and five IL-17 receptor (IL-17R)-family (IL-17RA-IL-17RE) members have been reported in mammalian cells. IL-17RA (known as IL-17R,IL-17AR,CD217) is a 128-158 kD type I transmembrane glycoprotein. IL-17R binds to IL-17A (also known as IL-17,and CTLA-8,is a hallmarker of Th17 cells) and IL-17F and mediates the proinflammatory signals through interaction with IL-17RC or IL-17RD. IL-17R has broad tissue distribution,including fibroblasts,epithelial cells,endothelial cells,stromal cells,B cells,T cells,NK cells,monocytes and granulocytes.

Gene Alias	Il17ra
Gene ID	23765
Uniport ID	Q60943
Protein Alias	Il17ra
Research Area	Immunology

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Mouse IL-17RA (Interleukin-17 receptor A) ELISA Kit (E-EL-M3118)

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