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Sensitivity | 0.1 ng/mL |
Detection Range | 0.16-10 ng/mL |
Sample Volume | 50 μL |
Total Assay Time | 2 h 30 min |
Reacitivity | Mouse |
Specificity | This kit recognizes Mouse GHRL in samples.No significant cross-reactivity or interference between Mouse GHRL and analogues was observed |
Recovery | 80%-120% |
Sample Type | Serum, plasma and other biological fluids |
Detection Method | Colorimetric method, ELISA, Competitive |
Assay Type | Competitive-ELISA |
Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
Storage | 2-8℃ |
Expiration Date | 12 months |
Uniport ID | Q9EQX0 |
Research Area | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction |
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1 Results
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1 Results
- The use of capsicum oleoresin microparticles to mitigate hepatic damage and metabolic disorders induced by obesity
IF:7.000
Journal:FOOD RESEARCH INTERNATIONAL(2024)
DOI:10.1016/j.foodres.2024.114932Reactivity:Mouse
Sample Type:plasma
- Ninjinyoeito ameliorates anorexia and changes in peptide YY and ghrelin levels of cisplatin-treated mice
IF:2.500
Journal:NEUROPEPTIDES(2024)
DOI:10.1016/j.npep.2024.102464Reactivity:Mouse
Sample Type:Plasma
- Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis
IF:64.500
Journal:CELL(2023)
DOI:10.1016/j.cell.2022.12.024Reactivity:Mouse
Sample Type:Serum
- Gut microbiota associated with appetite suppression in high-temperature and high-humidity environments
IF:11.100
Journal:EBioMedicine(2023)
DOI:10.1016/j.ebiom.2023.104918Reactivity:Mouse
Sample Type:serum
- Effects of Diet Macronutrient Composition on Weight Loss during Caloric Restriction and Subsequent Weight Regain during Refeeding in Aging Mice
IF:5.900
Journal:Nutrients(2023)
DOI:10.3390/nu15224836Reactivity:Mouse
Sample Type:Serum
- Activation of ectopic olfactory receptor 544 induces GLP-1 secretion and regulates gut inflammation
IF:10.245
Journal:Gut Microbes(2021)
DOI:10.1080/19490976.2021.1987782Reactivity:Mouse
Sample Type:plasma
- Heracleum dissectum Ledeb. ethanol extract attenuates metabolic syndrome symptoms in high-fat diet-induced obese mice by activating adiponectin/AMPK signaling
IF:4.451
Journal:Journal of Functional Foods(2021)
DOI:10.1016/j.jff.2021.104581Reactivity:Mouse
Sample Type:blood
- Asprosin, a novel glucogenic adipokine: a potential therapeutic implication in diabetes mellitus
IF:4.076
Journal:ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY(2021)
DOI:10.1080/13813455.2021.1894178Reactivity:Mouse
Sample Type:Serum
- Gymnaster Koraiensis Extract Alleviated Metabolic Syndrome Symptoms and Stimulated UCP1-Independent Energy Consumption via AMPK Activation in White Adipose Tissue
IF:5.309
Journal:MOLECULAR NUTRITION & FOOD RESEARCH(2020)
DOI:10.1002/mnfr.202000490Reactivity:Mouse
Sample Type:Serum
- Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels
IF:3.647
Journal:LIFE SCIENCES(2020)
DOI:10.1016/j.lfs.2020.117442Reactivity:Mouse
Sample Type:serum
Q1:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
Q2:Which variant of the spike protein does this kit detect? Do you have a kit specific to detect the spike protein for the omicron variant?
This kit is designed for the original strain of the new crown virus, and the omicron variant has not been verified. However, we have verified 26 recombinant variants of the SARS-CoV-2 spike protein through the kit. For more information, customers can refer to the kit instructions (https://file.elabscience.com/Manual/covid_19/E-EL-E605 .pdf).
Q3:What is the range of enzyme activity of your IL-2 freeze-dried powder
Currently our freeze-dried powder is a concentration unit with no information on the activity unit for the time being.
Q4:What is the principle of adding stop solution to stop color reaction in ELISA experiment?
On the one hand, the activity of HRP enzyme is destroyed and the catalytic function of HRP enzyme is lost. On the other hand, the final color changes due to a change in pH.
Q5:The absorbance of the cell supernatant is less than that of the sample diluent alone, so how to concentrate the sample?
The amount of medium can be reduced for subsequent drug administration and modeling.
Q6:My sample volume is small. Can I reduce some reagents proportionally?
No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.
Q7:May I ask which type of plate to choose when ELISA testing?
According to the different bottom, it is divided into flat bottom, U-shaped bottom, V-shaped bottom and so on. The index of refraction of the flat bottom is low, which is suitable for detection in the enzyme reader. According to the color can be divided into transparent, black, white. Transparent is commonly used for the most general enzyme-linked immunoassay.
Q8:Is the TGF-β1 ELISA Kit detecting the active form of TGF-β1 or the precursor?
The active TGF-β dimer was detected
Q9:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?
Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.
Q10:I got nothing on the IL-18 standard
The standard product is placed in the reagent bottle and then freeze-dried. You can first centrifuge the reagent bottle with 10000×g for 1min, and then directly observe the bottom or side wall of the reagent bottle.