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Mouse GFAP (glial fibrillary acidic protein) ELISA Kit (E-EL-M3111)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 609.00
48T $ 487.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 0.47 pg/mL
Detection Range 0.78-50 pg/mL
Sample Volume 100 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Mouse
Specificity This kit recognizes GFAP in samples.No significant cross-reactivity or interference between GFAP and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GFAP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GFAP , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GFAP . You can calculate the concentration of GFAP in the samples by comparing the OD of the samples to the standard curve.
GFAP is expressed exclusively in astrocytes in the central nervous system.The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Gene Alias Gfap
Gene ID 14580
Uniport ID P03995
Protein Alias Gfap
Research Area Cell Biology , Neuroscience
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