Mouse CRP Antibody Pair Set (E-KAB-0072)

C-Reactive Protein (CRP),also known as Pentraxin 1,is a non-glycosylated protein in the Pentraxin family that also includes Pentraxin 2/SAP and Pentraxin 3/TSG-14. CRP functions as a sensor and activator of the innate immune response (1). In humans,it is a major acute-phase protein,its circulating concentration is dramatically elevated at the onset of inflammation (2). In mice,however,serum CRP levels increase only slightly during inflammation,and the analogous acute phase role is filled by Pentraxin 2 (3). CRP assembles non-covalently into a 110-120 kDa cyclical pentamer (4). Mature human CRP shares 71% and 64% amino acid (aa) sequence identity with mouse and rat CRP,respectively (5). CRP binds and opsonizes apoptotic cells (6-8) as well as bacteria such as S. pneumoniae (9,10). It subsequently enhances the phagocytosis of these opsonized cells (6,8-10). CRP additionally binds several proteins in the complement cascade including C1q,C4BP,and Factor H (8,11-13). It enhances activation of the classical complement pathway and the deposition of C3b (9). In later stages of the response,CRP inhibits complement-mediated cell lysis through its binding to C4BP and Factor H (8,12). These interactions induce the upregulation of complement inhibitory proteins CD46,CD59,and CD55/DAF and inhibit assembly of the membrane attack complex (MAC) (8,14). CRP binds to Fc gamma RI,Fc gamma RIIA,and Fc gamma RIIB on macrophages and dendritic cells (15-17),and Fc receptors are required for the phagocytosis of CRP-opsonized target cells (6,10,18). CRP binding to Fc gamma RI induces Src activation which subsequently triggers the inhibitory Fc gamma RIIb and dampens the inflammatory response (15,19). CRP additionally promotes dendritic cell maturation and humoral immunity (10). In cardiovascular disease,CRP binds to oxidized LDL,exacerbates tissue damage in coronary artery infarction,and inhibits the repair of injured vascular endothelium (7,19,20).

Ptx1, Crp

Mouse

Detects Mouse CRP in ELISAs

ELISA

-20℃

12 months

This Antibody Pair Kit applies to the sandwich ELISA assay.