Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (E-BC-D012)
For research use only.
| Detection Principle |
Monoamine Oxidase (MAO) is a mitochondrial membrane binding enzyme, which is widely present in connective tissues and cell mitochondria of liver, kidney, small intestine, stomach, brain and other organs. MAO-A metabolizes neurotransmitters such as serotonin, dopamine, serotonin, epinephrine and norepinephrine. MOA inhibition increases levels of neurotransmitters and are used to treat depression, neurodegenerative diseases, and cardiovascular disease. The detection principle of this kit: MAO-A can catalyze the substrate to produce fluorescent substances. After adding inhibitors, the generation of fluorescent substances will be inhibited, and the effect of inhibitors will be determined according to the degree of inhibition. |
| Sample Type | Inhibitor |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Inhibitor Screening |
| Other Reagents Required | DMSO |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 5.0-8.9% | intra-assay CV: 0.7-5.0% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
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