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Monkey CTSB (Cathepsin B) ELISA Kit (E-EL-MK2300)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 682.00
48T $ 546.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 4.69 ng/mL
Detection Range 7.81-500 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Monkey
Specificity This kit recognizes CTSB in samples.No significant cross-reactivity or interference between CTSB and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CTSB . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CTSB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CTSB , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CTSB . You can calculate the concentration of CTSB in the samples by comparing the OD of the samples to the standard curve.
The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene.
Uniport ID Q4R5M2
Research Area Apoptosis/Tumor Suppressors/Cell Death , Cell Biology , Neurodegeneration , Neuroscience , Neuroscience Cell Markers , Protein Trafficking And Clearance , Stem Cells
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