MMP9 Monoclonal Antibody (E-AB-70247)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, Human liver cancer, Human liver cirrhosis, Human live, Rat paracancer, A549, Mouse lung, Mouse lung, Rat lung, Rat lung |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant protein corresponding to Mouse MMP9 |
| Abbre | MMP9 |
| Synonyms | 82 kDa matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, CLG 4B, CLG4B, Collagenase Type 4 beta, Collagenase type IV 92 KD, EC 3.4.24.35, GELB, Gelatinase 92 KD, Gelatinase B, Gelatinase beta, GelatinaseB, MANDP2, Macrophage gelatinase, Matrix m |
| Swissprot | |
| Calculated MW | 67/82 /92 kDa |
| Observed MW |
67/82/92 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 3.5 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Cardiovascular, Signal Transduction |
| Clone No. | 7C3A2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. |
Other Clones
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Unconjugated
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