Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	1.88 ng/mL
Detection Range	3.12-200 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Rat
Specificity	This kit recognizes CX3CL1 in samples.No significant cross-reactivity or interference between CX3CL1 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CX3CL1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CX3CL1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 51 nm. The OD value is proportional to the concentration of CX3CL1 . You can calculate the concentration of CX3CL1 in the samples by comparing the OD of the samples to the standard curve.

Background

CX3CL1 is a 373-amino acid macrocytokine protein that contains multiple domains and is the only known member of the CX3C chemokine family.It is also commonly referred to as fractalkine (in humans) and neurotactin (in mice).The polypeptide structure of CX3CL1 is different from the typical structure of other chemokines.For example, the spacing of characteristic n-terminal cysteine is different;There are three amino acids in CX3CL1 that distinguish the initial cysteine, none in the CC chemokines, and only one intermediate amino acid in the CXC chemokines.CX3CL1 is a long protein (373 amino acids in the human body) with an extended mucin-like stem and chemokine domain.Soluble CX3CL1 efficiently chemically adsorb T cells and monocytes, while the cells bind chemokines to promote the strong adhesion of leukocytes to activated endothelial cells, in which it is mainly expressed.

Gene Alias	Cx3cl1
Gene ID	89808
Uniport ID	O55145
Protein Alias	Cx3cl1
Research Area	Immunology , Neuroscience

Mini Sample Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mini Sample Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit (E-MSEL-R0002)

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