Mini Sample Mouse VEGFR1 ( Vascular Endothelial Growth Factor Receptor 1 ) ELISA Kit (E-MSEL-M0085)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VEGFR1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VEGFR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VEGFR1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VEGFR1 . You can calculate the concentration of VEGFR1 in the samples by comparing the OD of the samples to the standard curve.

The VEGF family includes five members, VEGFA (VEGFA165), VEGFB, VEGFC, VEGFD and placenta growth factor (PLGF). Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the regulation of angiogenesis, cell survival, cell migration, macrophage function, and chemotaxis.VEGFR1 is an angiogenic and anti-angiogenic factor depending on the ligand binding it (PLGF-1 inhibits angiogenesis, whereas VEGF and PLGF2 are pro-angiogenic).   Also, VEGFR1 mediates the angiostatic effect of pigment epithelium-derived factor (PEDF).