Mini Sample Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit (E-MSEL-M0005)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 3.21 pg/mL |
| Detection Range | 7.81-500 pg/mL |
| Sample Volume | 25 μL |
| Total Assay Time | 3 h 30 min |
| Reacitivity | Mouse |
| Specificity | This kit recognizes Mouse VEGF-A in samples.No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VEGF-A. You can calculate the concentration of Mouse VEGF-A in the samples by comparing the OD of the samples to the standard curve.
Background
| Uniport ID | Q00731 |
| Research Area | Cancer, Cardiovascular, Metabolism, Developmental Biology, Signal Transduction |
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