Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	32.81 pg/mL
Detection Range	54.69-3500 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes TRACP-5b in samples.No significant cross-reactivity or interference between TRACP-5b and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TRACP-5b . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TRACP-5b , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 42 nm. The OD value is proportional to the concentration of TRACP-5b . You can calculate the concentration of TRACP-5b in the samples by comparing the OD of the samples to the standard curve.

Background

Tartrate-resistant acid phosphatases (TRAPIACPS) are metallic enzymes belonging to the acid phosphatase group that catalyze the conversion of orthophosphate monomers into alcohols and orthophosphates.It is a ferriglycoprotein synthesized with a 35kd monomeric proenzyme.ACP5 is involved in bone resorption and is secreted by active osteoclasts.1 Dephosphorylation of osteopontin, a bone matrix protein, subsequently promotes osteoclast detachment and migration.In colorectal cancer, ovarian cancer, breast cancer, melanoma and other cancers,ACP5 expression is associated with tumor progression.In vitro experiments have shown that down-regulated expression of ACP5 can promote the proliferation and invasion of tumor cells in colorectal cancer cell lines.In senum, ACPS has been used as a marker for thyroid metastasis, and in addition, Acps removes the lysosomal protein as a recognition marker for mannose 6-phosphate.Tartrate-resistant acid phosphatases (TRAPIACPS) are metallic enzymes belonging to the acid phosphatase group that catalyze the conversion of orthophosphate monomers into alcohols and orthophosphates. It is a ferriglycoprotein synthesized with a 35kd monomeric proenzyme.

Gene Alias	Name Acp5 Synonyms T5ap, Trap
Gene ID	11433
Uniport ID	Q05117
Protein Alias	Name Acp5 Synonyms T5ap, Trap
Research Area	Cell Biology , Signal Transduction , Stem Cells

Mini Sample Mouse TRACP-5b (Tartrate Resistant Acid Phosphatase 5) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mini Sample Mouse TRACP-5b (Tartrate Resistant Acid Phosphatase 5) ELISA Kit (E-MSEL-M0117)

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