Mini Sample Mouse ST2 ( Syntenin 2 ) ELISA Kit (E-MSEL-M0100)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ST2 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ST2 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ST2 . You can calculate the concentration of ST2 in the samples by comparing the OD of the samples to the standard curve.

The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli , and may be involved in the function of helper T cells. This gene , interleukin 1 receptor , type I ( IL1R1 ) , interleukin 1 receptor , type II ( IL1R2 ) and interleukin 1 receptor-like 2 ( IL1RL2 ) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. Alternative splicing of this gene results in multiple transcript variants.