Mini Sample Mouse PTX3 ( Pentraxin 3 ) ELISA Kit (E-MSEL-M0097)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PTX3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PTX3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PTX3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PTX3 . You can calculate the concentration of PTX3 in the samples by comparing the OD of the samples to the standard curve.

PTX3 is part of the pentraxin family which is characterized by a cyclic multimeric structure. Penatraxins are acute phase proteins that act as pattern recognition receptors. PTX3 is a long pentraxin containing a C terminal domain similar to other members of the pentraxin family and a unique N terminal domain. Inflammatory signals stimulate PTX3 production by a variety of cells including mononuclear phagocytes, dendritic cells, fibroblasts, and endothelial cells.