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Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit (E-MSEL-M0116)

Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit - 1
  • Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit - 1
  • Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit - 2
  • Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit - 3
All Size Price Qty
96T $ 510.00
- +
48T $ 408.00
- +
24T $ 150.00
- +
96T*5 Inquire /
96T*10 Inquire /
Add to cart

For research use only.

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity 9.38 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Volume 25 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Mouse
Specificity This kit recognizes PF4 in samples.No significant cross-reactivity or interference between PF4 and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PF4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PF4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 41 nm. The OD value is proportional to the concentration of PF4 . You can calculate the concentration of PF4 in the samples by comparing the OD of the samples to the standard curve.
Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence. Chemotactic for neutrophils and monocytes via CCR1. Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis. In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition. Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity.
Gene Alias Pf4
Gene ID 56744
Uniport ID Q9Z126
Protein Alias Pf4
Research Area Cell Biology , Immunology
Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit - procedures
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