Mini Sample Mouse MFGE8 ( Milk Fat Globule EGF Factor 8 ) ELISA Kit

Size	Price	Qty
96T	$ 510.00
48T	$ 408.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Sensitivity	0.02ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes MFGE8 in samples.No significant cross-reactivity or interference between MFGE8 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MFGE8 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MFGE8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MFGE8 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MFGE8 . You can calculate the concentration of MFGE8 in the samples by comparing the OD of the samples to the standard curve.

Background

This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product , lactadherin , is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing , autoimmune disease , and cancer. Lactadherin can be further processed to form a smaller cleavage product , medin , which comprises the major protein component of aortic medial amyloid ( AMA ) . Alternative splicing results in multiple transcript variants.

Gene Alias	Mfge8
Gene ID	17304
Uniport ID	P21956
Protein Alias	Mfge8
Research Area	Cancer , Cardiovascular , Developmental Biology , Microbiology

Other Clones

{{antibodyDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Other Formats

{{formatDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

IF：{{item.impact}}

Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}
Reactivity：{{item.species}}

Sample Type：{{item.organization}}

Previous
{{ page }}
Next

Species

{{relatedElisaPage.numTotal}} Results

All

Human

Mouse

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}

Previous
{{ page }}
Next

All categories

Mini Sample Mouse MFGE8 ( Milk Fat Globule EGF Factor 8 ) ELISA Kit (E-MSEL-M0096)

Other Clones

Other Formats

Species