Mini Sample Mouse MCP-3 ( Monocyte Chemotactic Protein 3 ) ELISA Kit

Size	Price	Qty
96T	$ 510.00
48T	$ 408.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Sensitivity	0.17pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes MCP-3 in samples.No significant cross-reactivity or interference between MCP-3 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MCP-3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MCP-3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MCP-3 . You can calculate the concentration of MCP-3 in the samples by comparing the OD of the samples to the standard curve.

Background

CCL7, also known as MCP-3, was initially purified from cytokine-stimulated human osteosarcoma cell line (MG-63) conditioned medium. CCL7 has high similarity with CCL2/MCP-1 (71%), CCL8/MCP-2 (58%), and CCL13/MCP-4 (59%) at the amino acid level. Chemokines play a key role in inflammation and, in the initial phase, CXC chemokines chemoattract polymorphonuclear leukocytes (PMNs). The next step in the inflammatory processes is directed in part by CC chemokines, including the monocyte chemoattractant proteins (CCL2, CCL7, CCL8, and CCL13) that chemoattract additional leukocytes subsets.

Gene Alias	Ccl7
Gene ID	20306
Uniport ID	Q03366
Protein Alias	Ccl7
Research Area	Cell Biology , Immunology

Other Clones

{{antibodyDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Other Formats

{{formatDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

IF：{{item.impact}}

Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}
Reactivity：{{item.species}}

Sample Type：{{item.organization}}

Previous
{{ page }}
Next

Species

{{relatedElisaPage.numTotal}} Results

All

Human

Mouse

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}

Previous
{{ page }}
Next

All categories

Mini Sample Mouse MCP-3 ( Monocyte Chemotactic Protein 3 ) ELISA Kit (E-MSEL-M0101)

Other Clones

Other Formats

Species