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Mini Sample Mouse IL-9 (Interleukin 9) ELISA Kit (E-MSEL-M0118)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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Mini Sample Mouse IL-9 (Interleukin 9) ELISA Kit - 1
  • Mini Sample Mouse IL-9 (Interleukin 9) ELISA Kit - 1
  • Mini Sample Mouse IL-9 (Interleukin 9) ELISA Kit - 2
  • Mini Sample Mouse IL-9 (Interleukin 9) ELISA Kit - 3
All Size Price Qty
96T $ 510.00
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48T $ 408.00
- +
24T $ 150.00
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96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity 1.17 pg/mL
Detection Range 1.95-125 pg/mL
Sample Volume 25 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Mouse
Specificity This kit recognizes IL-9 in samples.No significant cross-reactivity or interference between IL-9 and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-9 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-9 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 43 nm. The OD value is proportional to the concentration of IL-9 . You can calculate the concentration of IL-9 in the samples by comparing the OD of the samples to the standard curve.
Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature mouse IL-9 shares 57% and 74% amino acid sequence identity with human and rat IL-9, respectively (14).
Gene Alias IL9
Gene ID 16198
Uniport ID P15247
Protein Alias IL9
Research Area Cell Biology , Signal Transduction , Stem Cells
Mini Sample Mouse IL-9 (Interleukin 9) ELISA Kit - procedures
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