Mini Sample Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) ELISA Kit (E-MSEL-M0104)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IGFBP-3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IGFBP-3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IGFBP-3 . You can calculate the concentration of IGFBP-3 in the samples by comparing the OD of the samples to the standard curve.

The Insulin-like Growth Factor (IGF) signaling system plays a central role in cellular growth,differentiation,and proliferation. IGFBP3 is the most abundant IGF binding protein in human serum and is a growth inhibitory,apoptosis-inducing molecule,capable of acting via IGF-dependent and IGF-independent mechanisms. It appears to function both by cell cycle blockade and the induction of apoptosis. IGFBP3 can be transported to the nucleus by an importin beta mediated mechanism,where it has been shown to interact with the retinoid X receptor alpha and possibly other nuclear elements. IGFBP3 antiproliferative signaling appears to require an active transforming growth factor-beta (TGF-beta) signaling pathway,and IGFBP3 stimulates phosphorylation of the TGF-beta signaling intermediates Smad2 and Smad3. IGFBP3 has IGF-independent roles in inhibiting cell proliferation in cancer cell lines. Nuclear transcription factor,retinoid X receptor (RXR)-alpha,and IGFBP3 functionally interact to reduce prostate tumor growth and prostate-specific antigen in vivo. Several clinical studies have proposed that individuals with IGFBP3 levels in the upper range of normal may have a decreased risk for certain common cancers. This includes evidence of a protective effect against breast cancer,prostate cancer,colorectal cancer,and lung cancer. Moreover,IGFBP3 inhibits insulin-stimulated glucose uptake into adipocytes independent of IGF.