Mini Sample Mouse ACE ( Angiotensin Ⅰ Converting Enzyme ) ELISA Kit (E-MSEL-M0078)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACE . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACE , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ACE . You can calculate the concentration of ACE in the samples by comparing the OD of the samples to the standard curve.

Angiotensin I-converting enzyme (ACE) catalyzes the conversion of angiotensin I into an active peptide angiotensin II, by the removal of two carboxy-terminal amino acids of angiotensin I. It is greatly expressed in endothelial and epithelial cells as a type I membrane grlycoprotein. A soluble form of ACE lacks the transmembrane domain and is present in biological fluids.