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Mini Sample Human sST2 (Soluble ST2) ELISA Kit (E-MSEL-H0015)

All Size Price Qty
96T $ 510.00
48T $ 408.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
Add to cart

For research use only.

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity 0.19 ng/mL
Detection Range 0.31-20 ng/mL
Sample Volume 25 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes sST2 in samples.No significant cross-reactivity or interference between sST2 and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to sST2 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for sST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain sST2 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of sST2 . You can calculate the concentration of sST2 in the samples by comparing the OD of the samples to the standard curve.
IL-1RL1/ST2 was initially identified in stimulated BALB/3T3 cells and is encoded by the IL-1RL1 gene that produces different isoforms by alternative splicing. In humans, four isoforms have been described: a membrane attached ST2L isoform and a soluble protein (sST2/ IL1RL1-a) that lacks the transmembrane and cytoplasmic domains, which acts as a natural antagonist of IL-33.
Gene Alias IL1RL1
Gene ID 9173
Uniport ID Q01638
Protein Alias IL1RL1
Research Area Immunology
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