Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	23.44 pg/mL
Detection Range	39.06-2500 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes OLFM4 in samples.No significant cross-reactivity or interference between OLFM4 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OLFM4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OLFM4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OLFM4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 38 nm. The OD value is proportional to the concentration of OLFM4 . You can calculate the concentration of OLFM4 in the samples by comparing the OD of the samples to the standard curve.

Background

OLFM4 (olfactomedin 4), also known as hGC-1(Human granulocyte colony-stimulating factor stimulated Clon1), is a protein containing the olfactomedin domain. OLFM4 is widely expressed in human stomach, small intestine, colon, bone marrow, prostate, pancreas and other normal tissues. The OLFM4 protein is known to be involved in the occurrence and development of inflammatory and immune diseases and has been shown to regulate important cellular processes such as cell growth and apoptosis. olff4 has also been shown to play a different role in regulating tumorigenesis and progression;

Gene Alias	OLFM4
Gene ID	10562
Uniport ID	Q6UX06
Protein Alias	OLFM4
Research Area	Immunology , Cancer

Mini Sample Human OLFM4 (Olfactomedin 4) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mini Sample Human OLFM4 (Olfactomedin 4) ELISA Kit (E-MSEL-H0038)

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