Mini Sample Human NGAL ( Neutrophil Gelatinase Associated Lipocalin ) ELISA Kit (E-MSEL-H0007)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NGAL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NGAL , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of NGAL . You can calculate the concentration of NGAL in the samples by comparing the OD of the samples to the standard curve.

NGAL, also known as lipocalin 2, LCN2, and siderocalin, is a small molecule composed of 178 amino acids.   It belongs to the lipocalin superfamily of 20 structurally related secreted proteins.     NGAL occurs predominantly in a monomeric form, with a small percentage occurring as a dimer or trimer.   It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils.   It is a critical component of innate immunity because its ability to bind iron-siderophore complexes limits bacterial growth.   Human NGAL is expressed at very low levels in human tissues, including kidney, trachea, lungs, stomach, and colon, and its expression increases greatly in the presence of inflammation and injured epithelia.   In addition to its role in innate immunity, it is a promising biomarker for acute kidney injury that is beginning to be used in clinical practice in addition to research studies.