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Mini Sample Human IFN-γ (Interferon Gamma) ELISA Kit (E-MSEL-H0030)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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Mini Sample Human IFN-γ (Interferon Gamma) ELISA Kit - 1
  • Mini Sample Human IFN-γ (Interferon Gamma) ELISA Kit - 1
  • Mini Sample Human IFN-γ (Interferon Gamma) ELISA Kit - 2
  • Mini Sample Human IFN-γ (Interferon Gamma) ELISA Kit - 3
All Size Price Qty
96T $ 510.00
- +
48T $ 408.00
- +
24T $ 150.00
- +
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity 14.06 pg/mL
Detection Range 23.44-1500 pg/mL
Sample Volume 25 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes IFN-γ in samples.No significant cross-reactivity or interference between IFN-γ and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IFN-γ . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IFN-γ , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 30 nm. The OD value is proportional to the concentration of IFN-γ . You can calculate the concentration of IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Human IFN-γ (interferon-γ) is an important immunomodulatory cytokine belonging to the Type II interferon family. It plays a key role in probiotic and adaptive immune responses, and has a wide range of biological functions, including antiviral, anti-tumor, immunomodulatory, and promoting cell proliferation and differentiation. IFN-γ is mainly produced by activated T cells (such as CD4+ and CD8+ T cells) and natural killer cells (NK cells). It is a non-covalent homologous dimer consisting of two 16 kDa subunits, which is modified by glycosylation to form a biologically active dimer. The molecular weight of IFN-γ is about 16 kDa, but higher molecular weight polymers can be formed under non-denatured conditions. IFN-γ, as an important immunomodulatory cytokine, plays a key role in both natural and adaptive immune responses. It not only has antiviral, anti-tumor and immunomodulatory functions, but also plays an important role in the occurrence and development of autoimmune diseases. Future studies will further reveal the specific mechanisms of IFN-γ in different diseases and provide new ideas for its clinical application.
Gene Alias IFNG
Gene ID 3458
Uniport ID P01579
Protein Alias IFNG
Research Area Immunology , Stem Cells , Cancer
Mini Sample Human IFN-γ (Interferon Gamma) ELISA Kit - procedures
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