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Mini Sample Human HO1  ( Heme Oxygenase 1 ) ELISA Kit (E-MSEL-H0020)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 510.00
48T $ 408.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 0.02ng/mL
Detection Range 0.31-20 ng/mL
Sample Volume 25 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes HO1 in samples.No significant cross-reactivity or interference between HO1 and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HO1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HO1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HO1 . You can calculate the concentration of HO1 in the samples by comparing the OD of the samples to the standard curve.
HMOX1 is a member of the heme oxygenase family.  Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc.  Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation.  The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.
Gene Alias HMOX1
Gene ID 3162
Uniport ID P09601
Protein Alias HMOX1
Research Area Cell Biology
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