Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes GAL3 in samples.No significant cross-reactivity or interference between GAL3 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GAL3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAL3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 32 nm. The OD value is proportional to the concentration of GAL3 . You can calculate the concentration of GAL3 in the samples by comparing the OD of the samples to the standard curve.

Background

Galectin-3 is a soluble β-galactoside binding protein, widely distributed in the heart, kidney, liver, lung and intestinal tissues and organs. Galectin-3 has a variety of biological functions, it can bind to a variety of ligands on the cell surface and extracellular matrix, and plays an important role in a series of physiological and pathological processes, including apoptosis, adhesion, proliferation, migration, inflammatory response, immune response, and fibrosis processes. In atherosclerosis, cardiac remodeling and myocardial fibrosis, Galectin 3 is abnormally overexpressed and secreted into the blood circulation. Galectin 3 can also enhance platelet aggregation, adhesion and retraction, and promote thrombosis. It is widely believed that it is involved in the growth and spread of cancer cells, can induce the secretion of protease in intestinal cancer cells, enhance the aggressiveness of cancer cells, and reduce the integrity of the epithelial mesenchyma between cancer cells. It is up-regulated in liver cancer and involved in the occurrence and development of liver cancer. It is also related to the occurrence and development of many diseases such as organ fibrosis, chronic inflammation and scar formation.

Gene Alias	LGALS3
Gene ID	3958
Uniport ID	P17931
Protein Alias	LGALS3
Research Area	Cell Biology , Neuroscience

Mini Sample Human GAL3 (Galectin 3) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mini Sample Human GAL3 (Galectin 3) ELISA Kit (E-MSEL-H0032)

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