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Mini Sample Human FGF23 ( Fibroblast Growth Factor 23) ELISA Kit (E-MSEL-H0012)

All Size Price Qty
96T $ 510.00
48T $ 408.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
Add to cart

For research use only.

Product Summary
Sensitivity 0.31pg/mL
Detection Range 62.50-4000 pg/mL
Sample Volume 25 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes FGF23 in samples.No significant cross-reactivity or interference between FGF23 and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGF23 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGF23 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FGF23 . You can calculate the concentration of FGF23 in the samples by comparing the OD of the samples to the standard curve.
Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
Gene Alias FGF23
Gene ID 8074
Uniport ID Q9GZV9
Protein Alias FGF23
Research Area Signal Transduction
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