Mini Sample Human DAO ( Diamine Oxidase ) ELISA Kit (E-MSEL-H0022)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DAO . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DAO , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of DAO . You can calculate the concentration of DAO in the samples by comparing the OD of the samples to the standard curve.

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide ( FAD ) as its prosthetic group. Its substrates include a wide variety of D-amino acids , but it is inactive on the naturally occurring L-amino acids. Its biological function is not known , it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice , it degrades D-serine , a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.