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Mini Sample Human BDNF ( Brain Derived Neurotrophic Factor ) ELISA Kit (E-MSEL-H0004)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 510.00
48T $ 408.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 28.82pg/mL
Detection Range 93.75-6000 pg/mL
Sample Volume 25 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes BDNF in samples.No significant cross-reactivity or interference between BDNF and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BDNF . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BDNF , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BDNF . You can calculate the concentration of BDNF in the samples by comparing the OD of the samples to the standard curve.
Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins) that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).  BDNF shares approximately 55% amino acid identity with other family members.  The amino acid sequence of mature BDNF is identical in all mammals examined.  All neurotrophins are noncovalently linked homodimers and initially produced as proneurotrophins.  These proneurotrophins have distinct biological activity different from their mature counterparts.  Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during development.  BDNF participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology.  BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.
Gene Alias BDNF
Gene ID 627
Uniport ID P23560
Protein Alias BDNF
Research Area Neuroscience , Stem Cells
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